![]() However, forensic scientists have continually worked towards reducing the amount of time and labor needed to generate these STR profiles (e.g., updates and developments in automation, direct amplification, polymerase chain reaction (PCR) protocols, etc.) as the demand on forensic resources, including trained laboratory personnel, continues to increase while reduced turnaround times are sought. Forensic laboratories currently use capillary electrophoresis (CE)-based technologies and workflows to generate STR profiles from biological evidence. Short tandem repeat (STR) typing is routinely used to associate or exclude individuals as possible contributors of biological evidence. Overall, the current Applied Biosystems RapidHIT ID Instrument with the ACE GlobalFiler Express sample cartridge was found to be a reliable tool for generation of STR profiles from reference-type buccal swabs. These validation studies included assessments of sensitivity, contamination, concordance, reproducibility and repeatability, stability, inhibition, mixtures, sample reprocessing, precision, and first-pass success rate. Thus, validation studies of a Rapid DNA workflow consisting of the Applied Biosystems RapidHIT ID Instrument and RapidLINK software with a focus on the ACE GlobalFiler Express Sample Cartridge and reference buccal swabs were performed in accordance with Scientific Working Group on DNA Analysis Methods Validation Guidelines. With a greater reliance on the system than on the analyst, validation data are especially needed to define the performance and limitations of commercially available Rapid DNA systems. ![]() Rapid DNA platforms are fully automated systems capable of processing DNA from biological samples and interpreting the results in approximately 90 minutes with minimal human intervention.
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